Journal Article 2,2ʼ,3,4,4ʼ,5,5ʼ-七塩素化ビフェニル(CB180)の動物肝ミクロゾームによる代謝
Metabolism of 2,2ʼ,3,4,4ʼ,5,5ʼ-Heptachlorobiphenyl (CB180) by Animal Liver Microsomes

太田, 千穂  ,  Ota, Chiho  ,  原口, 浩一  ,  加藤, 善久  ,  遠藤, 哲也  ,  木村, 治  ,  古賀, 信幸

106 ( 5 )  , pp.176 - 183 , 2015-05-25 , 福岡医学会
The in vitro metabolism of 2,2ʼ,3,4,4ʼ,5,5ʼ-heptachlorobiphenyl (CB180) was examined using liver microsomes of rats, guinea pigs and hamsters. Of liver microsomes from untreated animals, rats and guinea pigs produced one metabolite (M-1) with the activity of 1.2 and 18.1 pmol/hr/mg protein, respectively, but hamsters did not at all. Pretreatment of phenobarbital (PB) resulted in about 32-fold increase in rats, 4-fold increase in guinea pigs and an appearance of M-1 in hamsters (15 pmol/hr/mg protein). In addition, another metabolite (M-2) was formed only by liver microsomes of PB-treated guinea pigs. In contrast, pretreatment of 3-methylcholanthrene showed no metabolite in three animals. By comparison of the GC-MS data of the metabolites with synthesized authentic samples, M-1 and M-2 was determined to be 3ʼ-hydroxy (OH) -CB180 and 4’-OH-2,2ʼ,3,4,5,5ʼ-hexachlorobiphenyl (CB141), respectively. These results suggest that 3ʼ-OH-CB180 is a major metabolite and is formed by PB-inducible cytochrome P450 (CYP2B enzymes) in animals and also guinea pigs possess much higher activity to metabolize CB180 than rats and hamsters.

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