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Effect of Trinitrophenylation of Amino Groups on Biological Activities of Ricin DEffect of Trinitrophenylation of Amino Groups on Biological Activities of Ricin DAA00697606 |
"/Watanabe, Keiichi/"Watanabe, Keiichi ,
"/Funatsu, Gunki/"Funatsu, Gunki ,
"/渡辺, 啓一/"渡辺, 啓一 ,
"/船津, 軍喜/"船津, 軍喜
28
(
4
)
, pp.201
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211 , 1984-03 , Kyushu University , 九州大学農学部
ISSN:0023-6152
NII書誌ID(NCID):AA00697606
内容記述
Modification of amino groups with 2,4, ii-trinitrohenzenesulfonic acid (TNBS) remarkably reduced the toxicity of ricin D to mice, whereas cytoagglutinating and carbohydrate-binding activities were fully retained until the modification of 8 moles (73 %) of the free amino groups, indicating that the free amino groups exposed on the surface of ricin D molecule contribute to the toxic action but are not essential for the binding to oligosaccharides on cell surface. Three trinitrophenyl (TNP) amino groups out of eight in ricin D were found to be distributed to the A chain and five TNP-amino groups were deduced to be distributed to the B chain. The inhibitory activity of the TNP-ricin D to cell-free protein synthesis was more than 50% of that of native ricin D, indicating that amino groups in the A chain do not contribute much to the inhibition of protein synthesis. Digestibility of the TNP-ricin D by protease and its incorporation into cells also were the same as the native ricin D. These results demonstrate that the trinitrophenylation is one of the useful means of reducing the toxicity of ricin D without impairing the B chain’s ability to bind to the cell surface and the A chain’s ability to inhibit protein synthesis. A possible contribution of amino groups to the transport mechanism of ricin D to the cytoplasm are discussed in the results.
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https://qir.kyushu-u.ac.jp/dspace/bitstream/2324/23790/1/p201.pdf
